CDT Postgraduate Studentship in Healthcare Innovation (RCUK Digital Economy Programme grant number EP/G036861/1)
Active targeting of sonosensitive nanoparticles for cancer therapy
Standard chemotherapy drugs are limited by their lack of selectivity. Actively targeting cancer cells with nanoparticulate drug carriers has the potential to greatly improve drug delivery and consequently improve cancer treatment outcomes. I have optimised a two-step carbodiimide method to conjugate carboxylate modified polystyrene nanoparticles to murine epidermal growth factor (EGF). The resulting nanoparticles showed a specificity for IGROV-1 cancer cells expressing epidermal growth factor receptor (EGFR) that was significantly greater than for non-EGFR expressing A9 fibroblast cells. I have also conjugated Protein G to the surface of the carboxylate modified nanoparticles and used it as a bridging method between the nanoparticles and antibodies. This enables conjugation strategies to be compared. The ultimate aim is to apply the bioconjugation techniques described here to the targeting of novel sonosensitive nanoparticles for ultrasound-triggered drug release.